6NE2
Designed repeat protein in complex with Fz7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-12-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 57.610, 68.040, 86.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.220 - 1.299 |
R-factor | 0.1627 |
Rwork | 0.162 |
R-free | 0.18430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t44 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.299 | 1.299 |
Number of reflections | 83592 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 99.0 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M Sodium Acetate: HCl, pH 4.5, 2 M Ammonium Sulfate |