6NE1
Designed repeat protein in complex with Fz4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.846, 62.611, 86.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.431 - 3.011 |
| R-factor | 0.2404 |
| Rwork | 0.234 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bpb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.144 | 0.687 |
| Number of reflections | 5867 | 542 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium chloride, pH 6.3 and 20% PEG3,350 |
