6ND5

Crystal structure of the Thermus thermophilus 70S ribosome in complex with chloramphenicol and bound to mRNA and A-, P-, and E-site tRNAs at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	208.990, 448.290, 618.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	127.281 - 2.600
R-factor	0.2317
Rwork	0.229
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.003
RMSD bond angle	0.663
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	127.281	127.281	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.200	0.036	1.670
Rmeas	0.228	0.041	1.914
Total number of observations	7484694
Number of reflections	1733144	19611	128082
<I/σ(I)>	6.44	20.57	0.84
Completeness [%]	98.7	94.7	99.1
Redundancy	4.319	4.349	3.941
CC(1/2)	0.986	0.998	0.166

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME