6NCY

Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 3
Unit cell lengths	192.562, 192.562, 192.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.713 - 2.050
R-factor	0.1543
Rwork	0.154
R-free	0.18000
RMSD bond length	0.008
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.713	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.168	1.711
Rpim	0.028	0.460
Number of reflections	147997	14676
<I/σ(I)>	21.6
Completeness [%]	99.9	99.7
Redundancy	34.7	14.4
CC(1/2)	0.999	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M DL-malic acid, pH 7.0, 20% w/v PEG 3350