6NCE
Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | .979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.940, 102.080, 34.450 |
| Unit cell angles | 90.00, 102.65, 90.00 |
Refinement procedure
| Resolution | 30.570 - 2.598 |
| Rwork | 0.235 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c6y |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.542 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.570 | 2.691 |
| High resolution limit [Å] | 2.598 | 2.598 |
| Rmerge | 0.048 | 1.328 |
| Rmeas | 0.057 | 1.569 |
| Rpim | 0.031 | 0.825 |
| Number of reflections | 9664 | 958 |
| <I/σ(I)> | 12.6 | 1.09 |
| Completeness [%] | 96.6 | 94.47 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.996 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 0.1 M BisTris pH 5.4, 0.2 M MgCl2, 22% PEG 3350 |
