6NBP
Crystal Structure of a Sugar N-Formyltransferase from the Plant Pathogen Pantoea ananatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.928, 75.928, 87.591 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.18764 |
Rwork | 0.186 |
R-free | 0.22646 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.554 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.061 | 0.433 |
Number of reflections | 32264 | 4954 |
<I/σ(I)> | 13.1 | 2.1 |
Completeness [%] | 98.3 | 94.1 |
Redundancy | 7.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 13-16%PEG-3350, 2 mM folinic acid, 5 mM dTDP-Qui4N, 200 mM NaCl, 100 mM HEPES |