6NBK
Crystal structure of Arginase from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.022, 153.803, 83.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.444 - 1.910 |
| R-factor | 0.1631 |
| Rwork | 0.161 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cev.pdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.777 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.061 | 0.049 | 0.595 |
| Rmeas | 0.067 | 0.054 | 0.668 |
| Rpim | 0.027 | 0.022 | 0.295 |
| Total number of observations | 848033 | ||
| Number of reflections | 144946 | 7737 | 6575 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 99.0 | 99.7 | 91.2 |
| Redundancy | 5.9 | 6.2 | 4.4 |
| CC(1/2) | 0.993 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG3350 25%, 0.2M NH4Acte, 0.1M BisTr |
