6NBE
Ternary Complex of Ac-Alpha-Actin with Profilin and CoA-NAA80
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.469, 115.886, 132.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.350 - 2.000 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pbd |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.576 |
| Number of reflections | 54090 | 5172 |
| <I/σ(I)> | 30.2 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.3 | 25.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3 |
