6NB9

Amyloid-Beta (20-34) with L-isoaspartate 23

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TALOS ARCTICA
Temperature [K]	100
Detector technology	CMOS CAMERA
Collection date	2018-04-13
Detector	CETA 16M
Wavelength(s)	0.0251
Spacegroup name	P 1 21 1
Unit cell lengths	29.200, 4.870, 32.440
Unit cell angles	90.00, 101.90, 90.00

Refinement procedure

Resolution	5.955 - 1.050
R-factor	0.2022
Rwork	0.198
R-free	0.24580
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	5.955	5.955	1.100
High resolution limit [Å]	1.050	5.000	1.050
Rmerge	0.197	0.076	0.494
Rmeas		0.105	0.589
Rpim	0.224
Total number of observations	16529
Number of reflections	3946	10	315
<I/σ(I)>	3.76	5.92	1.4
Completeness [%]	82.7	17.2	53
Redundancy	4.189	2.2	3.092
CC(1/2)	0.984	0.989	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH	7.6	310	0.05M Tris-HCl, 0.15M NaCl, 1% DMSO