6NB1
Crystal structure of Escherichia coli ClpP protease complexed with small molecule activator, ACP1-06
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-30 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.985, 101.094, 155.194 |
| Unit cell angles | 90.00, 98.29, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.900 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yg6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.002 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | |
| Number of reflections | 225772 | 6914 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 51-63% MPD 0.1 M sodium acetate pH 5.0 |
