6NA6

Serial Femtosecond X-ray Crystallography Structure of ECR in complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2017-05-23
Detector	MPCCD
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	109.760, 78.050, 138.000
Unit cell angles	90.00, 107.76, 90.00

Refinement procedure

Resolution	31.817 - 2.100
R-factor	0.275
Rwork	0.274
R-free	0.33340
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	131589
<I/σ(I)>	6.65
Completeness [%]	100.0
Redundancy	43.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	MIDAS screen