6NA3

Crystal Structure of Apo-form of ECR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	78.100, 153.000, 202.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.966 - 1.800
R-factor	0.1994
Rwork	0.199
R-free	0.24200
RMSD bond length	0.010
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.966	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	223907
<I/σ(I)>	12.32
Completeness [%]	91.5
Redundancy	6.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.1M TRIS ph 8.0 20% w/v Poly(acrylic acid sodium salt) 5100 30% Glycerol