6N9T
Structure of a peptide-based photo-affinity cross-linker with Herceptin Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-08-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.112, 60.850, 68.167 |
Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
Resolution | 44.224 - 2.576 |
R-factor | 0.2309 |
Rwork | 0.229 |
R-free | 0.26510 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.585 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12-2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.585 |
High resolution limit [Å] | 2.576 | 2.576 |
Number of reflections | 31910 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 100.0 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 100mM sodium acetate pH=5.6, 12%(w/v) PEG 1000 |