6N9N
Crystal structure of murine GSDMD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.420, 86.840, 83.540 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 46.013 - 3.300 |
| R-factor | 0.2639 |
| Rwork | 0.261 |
| R-free | 0.32070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ao3 5b5r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.013 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 14323 | 1037 |
| <I/σ(I)> | 15.68 | 1.2 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.52 | 3.75 |
| CC(1/2) | 0.999 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350, 0.1M Bis-Tris pH 6.5, and 10 mM DTT |
