6N9G

Crystal Structure of RGS7-Gbeta5 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2017-10-17
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.323, 162.760, 95.295
Unit cell angles	90.00, 98.36, 90.00

Refinement procedure

Resolution	37.535 - 2.129
R-factor	0.1764
Rwork	0.174
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pbi
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.280	2.480
High resolution limit [Å]	2.129	2.130
Rmerge	0.064	0.817
Rpim	0.039	0.500
Number of reflections	54345	2718
<I/σ(I)>	14.6
Completeness [%]	99.7
Redundancy	3.8
CC(1/2)	0.999	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.25	277	0.1M sodium malonate (pH 6.25) and 10% w/v Polyethylene glycol 3,350