6N9B
FtsY-NG ultra high-resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-26 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.683, 76.180, 108.786 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 44.200 - 1.219 |
| R-factor | 0.1565 |
| Rwork | 0.155 |
| R-free | 0.17880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.263 |
| High resolution limit [Å] | 1.219 | 1.219 |
| Rmerge | 0.029 | |
| Number of reflections | 165619 | |
| <I/σ(I)> | 14.75 | |
| Completeness [%] | 98.7 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297.15 | 0.1 M Bis-Tris pH 6.6, 24% PEG 2000 MME, 200 mM KBr. |
