6N7J
BDBV223 Fab bound to synthetic peptide of Bundibugyo virus Glycoprotein Stalk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2016-05-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.452, 191.580, 63.362 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 48.356 - 3.684 |
| R-factor | 0.2289 |
| Rwork | 0.228 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6MAM chains H & L |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.356 | 3.820 |
| High resolution limit [Å] | 3.680 | 3.680 |
| Rmerge | 0.310 | 0.680 |
| Rpim | 0.190 | 0.430 |
| Number of reflections | 12104 | 1176 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 97.1 | 96.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.950 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 20% w/v PEG8000, 0.2 M magnesium acetate, 0.1 M sodium cacodylate:HCl, pH 6.5 |
