6N7E

Crystal structure of the cytosolic domain of human CNNM2 in complex with AMP-PNP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	290.582, 111.139, 103.519
Unit cell angles	90.00, 101.01, 90.00

Refinement procedure

Resolution	48.760 - 3.500
Rwork	0.282
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p1o 6dj3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12-2829)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	36991	683
<I/σ(I)>	17.6	0.5
Completeness [%]	99.8	100
Redundancy	3.4	3.4
CC(1/2)		0.148

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M Bis-Tris, pH 6.5, 0.5 M magnesium formate, 5 mM AMP-PNP