6N73
Crystal Structure of ATPase delta1-79 Spa47 R271A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.550 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.199, 154.117, 54.905 |
Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
Resolution | 32.295 - 2.398 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5swj |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.111 | 0.079 | 0.652 |
Rmeas | 0.123 | 0.087 | 0.732 |
Rpim | 0.050 | 0.036 | 0.326 |
Total number of observations | 150622 | ||
Number of reflections | 25991 | 2679 | 2430 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 96.4 | 97.6 | 90.6 |
Redundancy | 5.8 | 5.9 | 4.5 |
CC(1/2) | 0.995 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 298 | Tris, Ammonium Acetate, Lithium Sulfate, PEG 4000, MPD |