6N6V
OXA-23 mutant F110A/M221A low pH form meropenem complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.114, 44.012, 46.222 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.957 - 1.550 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.19170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jf5 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.500 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.072 | 1.078 |
Rpim | 0.026 | 0.414 |
Number of reflections | 41640 | 1964 |
<I/σ(I)> | 17.4 | |
Completeness [%] | 99.6 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 293 | 0.06 M citric acid, 0.04 M bis-Tris propane, pH 4.1, 16% PEG3350 |