6N6K
Human REXO2 bound to pAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.806, 87.423, 124.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.384 - 1.418 |
| R-factor | 0.1536 |
| Rwork | 0.153 |
| R-free | 0.17240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.806 | 45.806 | 1.490 |
| High resolution limit [Å] | 1.418 | 4.480 | 1.420 |
| Rmerge | 0.036 | 0.724 | |
| Rmeas | 0.078 | 0.039 | 0.779 |
| Rpim | 0.028 | 0.014 | 0.282 |
| Total number of observations | 746585 | ||
| Number of reflections | 95286 | 3300 | 13642 |
| <I/σ(I)> | 15.7 | 14.3 | 1.1 |
| Completeness [%] | 99.8 | 99.8 | 98.9 |
| Redundancy | 7.8 | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2 M sodium malonate (pH 5.5) and 15-20% polyethylene glycol 3350, 25% glycerol |
