6N61

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Capistruin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 41 21 2
Unit cell lengths	172.888, 172.888, 385.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.445 - 3.253
R-factor	0.2735
Rwork	0.273
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljz
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.370
High resolution limit [Å]	3.250	3.250
Rpim	0.087	1.385
Number of reflections	92082	8850
<I/σ(I)>	15.16	0.76
Completeness [%]	99.0	98
Redundancy	20.5	8.3
CC(1/2)	0.996	0.102

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	0.1 M HEPES, pH 6.8, 0.2 M magnesium chloride, 7% w/v PEG3350, 4% v/v glycerol, 4% v/v ethylene glycol