6N5Y

Crystal structure of the SNX5 PX domain in complex with the CI-MPR (space group P212121 - Form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97803
Spacegroup name	C 2 2 21
Unit cell lengths	33.063, 134.584, 98.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 2.260
R-factor	0.2116
Rwork	0.209
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.330
High resolution limit [Å]	2.260	2.260
Number of reflections	10740
<I/σ(I)>	16.7
Completeness [%]	99.8
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	75% of 0.2 M (NH4)SO4, 0.1 M MES pH 6.5, 30% (w/v) PEG5000 MME + 15% glycerol + 30% v/v (+/-)-2-Methyl-2,4-pentanediol