6N5S
Structure of Human pir-miRNA-320b-2 Apical Loop and One-base-pair Stem Fused to the YdaO Riboswitch Scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.560, 114.560, 115.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.137 - 2.802 |
| R-factor | 0.1789 |
| Rwork | 0.176 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qk8 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.137 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.062 | 1.730 |
| Rpim | 0.020 | 0.499 |
| Number of reflections | 21893 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 9.92 | 9.95 |
| CC(1/2) | 0.998 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 1.9 M (NH4)2SO4, 0.2 M Li2SO4, and 0.1 M HEPES pH 7.4 |
