6N5I

FtsY-NG high-resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	34.753, 76.207, 108.989
Unit cell angles	90.00, 93.85, 90.00

Refinement procedure

Resolution	34.670 - 1.498
R-factor	0.1352
Rwork	0.133
R-free	0.18180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.670	1.552
High resolution limit [Å]	1.498	1.498
Rmerge	0.023	0.132
Rmeas	0.032	0.186
Rpim	0.023	0.132
Number of reflections	88464
<I/σ(I)>	18.22
Completeness [%]	97.3	96.78
Redundancy	1.8	1.7
CC(1/2)	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	297.15	0.1 M Bis-Tris pH 5.8, 26.5% PEG 2000 MME, 175 mM KBr