6N53

Crystal structure of human uridine-cytidine kinase 2 complexed with 2'-azidouridine monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	198
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	F 2 2 2
Unit cell lengths	89.896, 142.404, 248.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.818 - 2.700
R-factor	0.2003
Rwork	0.196
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uei
RMSD bond length	0.002
RMSD bond angle	0.755
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmeas	0.084	0.492
Rpim	0.028	0.195
Number of reflections	21912	958
<I/σ(I)>	29.3	1.85
Completeness [%]	98.0	85.5
Redundancy	8	5.3
CC(1/2)		0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Na/K phosphate, 22% PEG 3350