6N4O
Human Argonaute2-miR-122 bound to a seed and supplementary paired target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.885570 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.132, 66.328, 247.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.899 |
| R-factor | 0.2099 |
| Rwork | 0.207 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w5o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 3.060 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.107 | 0.505 |
| Rpim | 0.044 | 0.213 |
| Number of reflections | 24197 | |
| <I/σ(I)> | 13.5 | 4 |
| Completeness [%] | 96.8 | 96.3 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 26% MPD, 5% PEG 3350, 50 mM imidazole pH 8 |
