6N3U
MicroED Structure of the CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Wavelength(s) | 0.0251 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.356, 122.983, 81.820 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 19.946 - 2.900 |
| R-factor | 0.2401 |
| Rwork | 0.236 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i4t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2747) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.180 | 0.860 |
| Number of reflections | 12951 | |
| <I/σ(I)> | 3.7 | 1.1 |
| Completeness [%] | 83.4 | 82.3 |
| Redundancy | 7.5 | 7.5 |
| CC(1/2) | 0.960 | 0.100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | 0.1 M Bis-Tris Propane, 1.1 M LiSO4 |
