6N3J

MicroED Structure of the CTD-SP1 fragment of HIV-1 Gag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Wavelength(s)	0.0251
Spacegroup name	C 1 2 1
Unit cell lengths	70.614, 122.552, 78.704
Unit cell angles	90.00, 96.55, 90.00

Refinement procedure

Resolution	19.863 - 3.000
R-factor	0.2576
Rwork	0.254
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i4t
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_2747)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.500	3.200
High resolution limit [Å]	3.000	3.000
Rpim	0.255	1.324
Number of reflections	10480
<I/σ(I)>	3	0.7
Completeness [%]	79.8	78.7
Redundancy	5.7	5.7
CC(1/2)	0.900	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	0.1 M Bis-Tris Propane, 1.1 M LiSO4