6N2U

IL-8 Structure from Bacterial Expression Source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	39.701, 39.701, 89.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.120 - 1.254
R-factor	0.1905
Rwork	0.187
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d14
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.120	1.299
High resolution limit [Å]	1.254	1.254
Rmerge	0.026	0.322
Rmeas	0.037	0.322
Rpim	0.026	0.752
Number of reflections	13693	122
<I/σ(I)>	15.99
Completeness [%]	58.8	5.34
Redundancy	2
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.4 M Ammonium Phosphate dibasic, 0.1 M Tris HCl pH 8.5