6N2O

2-oxoglutarate:ferredoxin oxidoreductase from Magnetococcus marinus with 2-oxoglutarate, coenzyme A and succinyl-CoA bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	86.407, 100.532, 202.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.006 - 2.824
R-factor	0.2148
Rwork	0.212
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n2n
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	38629	2935
<I/σ(I)>	10.9
Completeness [%]	90.7
Redundancy	8.1
CC(1/2)		0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	27% (w/v) PEG 4000 and 0.08 M sodium cacodylate pH 5.5