6N2N

Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from Magnetococcus marinus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	86.282, 123.161, 162.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.152 - 1.937
R-factor	0.1698
Rwork	0.168
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wbx 5c4i 3g2e
RMSD bond length	0.005
RMSD bond angle	0.710
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.010
High resolution limit [Å]	1.937	1.937
Number of reflections	125663	11651
<I/σ(I)>	7.7
Completeness [%]	97.6
Redundancy	4
CC(1/2)		0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	14% (w/v) PEG 8000, 0.21-0.28 M (NH4)2SO4, and 0.10 M MES pH 6.5