6N2F
Meso-Diaminopimelate Decarboxylase from Arabidopsis thaliana (Isoform 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.381, 107.989, 80.929 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 46.170 - 2.270 |
| R-factor | 0.2023 |
| Rwork | 0.199 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p3e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.556 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.770 | 39.770 | 2.430 |
| High resolution limit [Å] | 2.350 | 9.100 | 2.350 |
| Rmerge | 0.195 | 0.050 | 0.975 |
| Rmeas | 0.229 | 0.058 | 1.156 |
| Rpim | 0.118 | 0.030 | 0.615 |
| Total number of observations | 132573 | 2360 | 11672 |
| Number of reflections | 35978 | 647 | 3454 |
| <I/σ(I)> | 5.5 | 18.7 | 1.3 |
| Completeness [%] | 99.7 | 98.9 | 97.9 |
| Redundancy | 3.7 | 3.6 | 3.4 |
| CC(1/2) | 0.987 | 0.996 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 8 mg/ml protein in buffer containing 0.2 mM PLP and 5 mM lysine mixed in a 1:1 ratio with a reservoir solution consisting of 0.28 M sodium acetate, 0.1 M bis-tris propane pH 7.2, 25%(w/v) PEG 3350 |
