6N2B
The Crystal Structure of Caldicellulosiruptor kristjanssonii Tapirin C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-21 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.093, 98.317, 196.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.425 - 2.650 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wa0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.986 |
| Data reduction software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 38585 | 3969 |
| <I/σ(I)> | 4.89 | 1.03 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.23 | 4.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M citric acid pH 3.0 to 4.0 and 10% to 15% w/v polyethylene glycol 3350 |
