6N1G

Crystal structure of Aquaglyceroporin AQP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-09
Detector	DECTRIS PILATUS 6M-F
Spacegroup name	F 41 3 2
Unit cell lengths	385.658, 385.658, 385.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.711 - 3.995
R-factor	0.2327
Rwork	0.232
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d9s
RMSD bond length	0.007
RMSD bond angle	1.029
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3235: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.711	4.137
High resolution limit [Å]	3.995	3.995
Number of reflections	18776
<I/σ(I)>	38.77
Completeness [%]	88.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	30% w/v polyethylene glycol 400, 100 mM MOPS pH 7.0, 100 mM NaCl.