6N10
Crystal structure of Arabidopsis thaliana mevalonate 5-diphosphate decarboxylase 1 complexed with (R)-MVAPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.630, 106.630, 166.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.168 - 2.300 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d4j |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.457 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | Aimless (0.2.7) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.315 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.121 | 0.536 |
| Number of reflections | 42293 | 2744 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 97.8 | 96 |
| Redundancy | 10.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.1 M sodium citrate tribasic pH 5.6 0.2 M potassium sodium tartrate 2.0 M ammonium sulfate 5mM (R)-MVAPP |
