6MZN

Zebrafish betaglycan orphan domain structure from tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-14
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	62.940, 62.940, 114.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.330 - 2.380
R-factor	0.202
Rwork	0.198
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mzp
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.110	2.470
High resolution limit [Å]	2.380	2.380
Rmerge	0.080	1.784
Rmeas	0.093	2.104
Rpim	0.046	0.786
Number of reflections	17750	1873
<I/σ(I)>	10.6	1.1
Completeness [%]	99.4	99.8
Redundancy	7.5	6.8
CC(1/2)	0.998	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	8% v/v PEG 5K mono methyl ether, 4% w/v PEG 20K, 0.1M MES pH 6.5