6MZF

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	218.800, 107.650, 282.740
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	96.592 - 4.400
R-factor	0.2331
Rwork	0.225
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.566
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.592	96.592	4.640
High resolution limit [Å]	4.400	13.910	4.400
Rmerge		0.061	0.478
Rmeas	0.198	0.069	0.675
Rpim	0.112	0.038	0.386
Number of reflections	80099	2576	11567
<I/σ(I)>	4.9	6.1	1.4
Completeness [%]	95.4	92.3	94.8
Redundancy	2.9	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000