6MZE

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	218.480, 106.150, 282.230
Unit cell angles	90.00, 90.39, 90.00

Refinement procedure

Resolution	59.453 - 3.600
R-factor	0.2059
Rwork	0.202
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.453	59.453	3.790
High resolution limit [Å]	3.600	11.380	3.600
Rmerge		0.036	0.650
Rmeas	0.199	0.048	1.007
Rpim	0.115	0.028	0.581
Number of reflections	142673	4586	21124
<I/σ(I)>	4.8	14.4	1.2
Completeness [%]	95.0	92	96.7
Redundancy	2.7	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000