6MYV

Sialidase26 co-crystallized with DANA-Gc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	P 1
Unit cell lengths	85.645, 89.753, 95.806
Unit cell angles	64.70, 75.65, 88.58

Refinement procedure

Resolution	48.500 - 2.200
R-factor	0.232
Rwork	0.231
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q6k
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	126317
<I/σ(I)>	6
Completeness [%]	98.3
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	20% PEG 6000, 0.1 M Tris-HCl pH 8.0