6MYQ

Avian mitochondrial complex II with ferulenol bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	69.910, 84.553, 290.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 1.970
R-factor	0.2088
Rwork	0.208
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_3150)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	2.030
High resolution limit [Å]	1.970	1.970
Rmerge	0.085	0.905
Rmeas		1.159
Rpim		0.710
Number of reflections	115414	7077
<I/σ(I)>	5.7	0.9
Completeness [%]	94.1	70.3
Redundancy	3.22	2.04
CC(1/2)		0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with ferulenol after crystallization