6MYP

Avian mitochondrial complex II with TTFA (thenoyltrifluoroacetone) bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97430
Spacegroup name	P 21 21 21
Unit cell lengths	69.951, 84.303, 290.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.360 - 2.100
R-factor	0.235
Rwork	0.235
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.002
RMSD bond angle	0.510
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((DEV_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.360	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.114	0.636
Number of reflections	100834
<I/σ(I)>	6.9	2.2
Completeness [%]	99.6	99.6
Redundancy	4.7	4.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	48 g/L PEG3350, 15 mL/L PEG400, 24 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 1.6 mM manganese chloride, 0.64 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, TTFA