6MYO
Avian mitochondrial complex II with flutolanyl bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.978483 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.637, 83.783, 289.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.640 - 2.200 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((DEV_3150: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.650 | 2.240 |
High resolution limit [Å] | 2.173 | 2.170 |
Rmerge | 0.118 | 1.154 |
Number of reflections | 80438 | |
<I/σ(I)> | 9.81 | 1.6 |
Completeness [%] | 90.1 | 43.7 |
Redundancy | 9.7 | 5.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside |