6MYK

Pleurotus ostreatus OstreolysinA mutant E69A with Bis-Tris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97903
Spacegroup name	C 1 2 1
Unit cell lengths	69.857, 86.684, 92.878
Unit cell angles	90.00, 99.13, 90.00

Refinement procedure

Resolution	49.265 - 1.800
R-factor	0.1952
Rwork	0.194
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ov8
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.039	0.024	0.438
Rmeas	0.043	0.027	0.499
Rpim	0.018	0.011	0.233
Number of reflections	47399	2552	1701
<I/σ(I)>	9.6
Completeness [%]	93.4	97.7	68.4
Redundancy	5.2	5.7	3.9
CC(1/2)		0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris, 0.125 M lithium sulfate, 25% (w/v) PEG3350, 25% ethylene glycol