6MYE

Crystal structure of human Scribble PDZ1 domain in complex with internal PDZ binding motif of Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-08-13
Detector	RDI CMOS_8M
Wavelength(s)	1.0003
Spacegroup name	P 43 21 2
Unit cell lengths	67.890, 67.890, 44.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.005 - 1.100
R-factor	0.1983
Rwork	0.198
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w4f
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.052	0.448
Rpim		0.409
Number of reflections	42446
<I/σ(I)>	26.7	1.8
Completeness [%]	100.0	100
Redundancy	15.6	12.8
CC(1/2)		0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	60% (v/v) Tacsimate pH7.0