6MXV

The crystal structure of a rhodanese-like family protein from Francisella tularensis subsp. tularensis SCHU S4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97818
Spacegroup name	P 1 21 1
Unit cell lengths	48.721, 111.108, 50.339
Unit cell angles	90.00, 93.49, 90.00

Refinement procedure

Resolution	48.600 - 1.780
R-factor	0.1592
Rwork	0.158
R-free	0.18840
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.958
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.061	0.490
Rmeas	0.069	0.560
Rpim	0.031	0.266
Number of reflections	50307	2503
<I/σ(I)>	22.3	3.67
Completeness [%]	99.0	99
Redundancy	4.4	3.8
CC(1/2)		0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	PEG3350 25%, 0.2M MgCl2, 0.1M Tris