6MTZ

Crystal structure of an uncharacterized protein from Helicobacter pylori G27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-14
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	60.730, 66.740, 200.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.943 - 2.150
R-factor	0.1777
Rwork	0.175
R-free	0.22790
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.943	44.943	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.097	0.049	0.606
Rmeas	0.101	0.051	0.647
Number of reflections	45317	594	3281
<I/σ(I)>	17.92	39.48	3.49
Completeness [%]	100.0	97.2	100
Redundancy	13.491	11.719	8.285
CC(1/2)	0.999	0.999	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	Microlytic MCSG1 screen, F3: 200mM Ammonium citrate dibasic pH 5, 20% PEG3350: HepyC.18480.a.B11.PW38477 at 31.62mg/ml. For phasing, a crysta was incubated for 10sec each in reservoir 10% 2.5M NaI in EG and 20% 2.5M NaI in EG (500mM final NaI concentration), and directly vitrified. Tray 301766f3, puck OVZ1-5