6MSF
F6 APTAMER MS2 COAT PROTEIN COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 287.580, 287.580, 652.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.000 - 2.800 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.20100 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.186 | 0.369 |
| Total number of observations | 313760 * | |
| Number of reflections | 169875 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 68.0 | 42.8 * |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 30 * | PROTEIN IN 1.25% OR 1.5% PEG 8000, 0.1M NA PHOSPHATE PH 7.4 AND 0.02% NA AZIDE WAS EQUILIBRATED AGAINST 0.35M OR 0.4M NA PHOSPHATE PH 7.4, 0.02% NA AZIDE AT 30O OR 37O C. WASHED CRYSTALS WERE SOAKED IN 2MG/ML RNA. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.75 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 1.25 (%(w/v)) | or 1.5%(w/v) |
| 3 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 4 | 1 | reservoir | sodium phosphate | 0.35 (M) | or 0.4M |
