6MS1
Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.338, 51.102, 57.051 |
| Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
| Resolution | 39.503 - 1.350 |
| R-factor | 0.1788 |
| Rwork | 0.178 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vwk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.673 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.503 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.072 | 0.448 |
| Rmeas | 0.087 | 0.606 |
| Rpim | 0.039 | 0.284 |
| Number of reflections | 38341 | 1786 |
| <I/σ(I)> | 7 | 1.5 |
| Completeness [%] | 97.5 | 91.3 |
| Redundancy | 4.6 | 4.1 |
| CC(1/2) | 0.996 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Potassium formate, 20% PEG 3350 |
