6MRP
Structure of the Bovine p85a BH domain R228E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.187, 92.697, 93.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.559 - 2.403 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22470 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | wild type protein model |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.085 | 0.601 |
| Number of reflections | 29393 | 1454 |
| <I/σ(I)> | 33 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 1.5 M Li2SO4, 4% glycerol, 0.1 Sodium Cacodylate buffer |
